Fluorescent proteins for multiphoton and super-resolution imaging
نویسنده
چکیده
87 Progress in fluorescence imaging is enabling the study of biological events at unprecedented detail, thanks to novel microscopy techniques that provide imaging with nanoscale resolution. 3D imaging of living cells within intact tissues, organs, and whole animals, is accessible through multiphoton excitation. The remarkably rich photophysics of Fluorescent Proteins (FPs) makes them flexible enough to be used in conjunction with these advanced spectroscopic approaches. Here we report on our progress in understanding FPs multiphoton excitation spectra and in unraveling the photophysics behind photoactivation/deactivation of some FPs, a property enabling their use as fluorophores for nano-resolution imaging. This analysis allows us to develop new reversibly switchable FP mutants.
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